AskDefine | Define citrate

Dictionary Definition

citrate v : cause to form a salt or ester of citric acid

User Contributed Dictionary



  1. Any salt or ester of citric acid.


salt or ester of citric acid
  • Italian: citrato
  • Swedish: citrat

Extensive Definition

A citrate can refer either to the conjugate base of citric acid, (C3H5O(COO)33−), or to the esters of citric acid. An example of the former, a salt is trisodium citrate; an ester is trimethyl citrate.

Other citric acid ions

Since citric acid is a multifunctional acid, intermediate ions exist, hydrogen citrate ion, HC6H5O72− and dihydrogen citrate ion, H2C6H5O7−. These may form salts as well, called acid salts.


Salts of the hydrogen peroxide citrate ions are weakly acidic, while salts of the citrate ion itself (with an inert cation such as sodium ion) are weakly basic.


Citrate is a key component in the commonly used SSC 20X hybridization buffer. There exists authoritative literature (Maniatis) that incorrectly instructs the preparation of this buffer to include 3M NaCl and 0.3M Sodium Citrate, to be titrated up with NaOH to a pH of 7. When the two components are actually mixed together, the pH is slightly basic. Therefore, the pH of the solution should instead be titrated down to 7 with HCl.
Citric acid can act as a mild chelating agent.


TCA cycle

Citrate is an intermediate in the TCA (Krebs) Cycle. After the pyruvate dehydrogenase complex forms acetyl CoA, from pyruvate and five cofactors (Thiamine pyrophosphate, lipoamide, FAD, NAD+, and CoA), citrate synthase catalyzes the condensation of oxaloacetate with Acetyl CoA to form citrate. Citrate continues in the TCA cycle via aconitase with the eventual regeneration of oxaloacetate, which can combine with another molecule of acetyl CoA and continue cycling.
See also TCA cycle

Role in Glycolysis

High concentration of citrate can inhibit phosphofructokinase, the pace-maker of glycolysis.
citrate in Spanish: Citrato
citrate in Polish: Cytryniany
citrate in Portuguese: Citrato
citrate in Russian: Цитрат
citrate in Sundanese: Sitrat
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